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TDF100A
Sigma
Total Dietary Fiber Assay Kit
sufficient for ~100 assays
Description
| Other Notes | Reagents for the determination of total dietary fiber. |
| Principle | Uses a combination of enzymatic and gravimetric methods. This procedure is based on the method published in Official Methods of Analysis, 15th ed., AOAC, Arlington, VA, Vol. II, Sec. 985.29, 1105 (1990). |
| Application | This assay determines the total dietary fiber content of food using a combination of enzymatic and gravimetric methods. Samples of dried, fat-free foods are gelatinized with heat stable α-amylase and then enzymatically digested with protease and amyloglucosidase to remove the protein and starch present in the sample. Ethanol is added to precipitate the soluble dietary fiber. The residue is then filtered and washed with ethanol and acetone. After drying, the residue is weighed. Half of the samples are analyzed for protein and the others are ashed. Total dietary fiber is the weight of the residue less the weight of the protein and ash. |
Properties
| usage | sufficient for ~100 assays |
| storage temp. | 2-8°C |
Safety
| Hazard Codes | Xn |
| Risk Statements | 37/38-41-42-48/20 |
| Safety Statements | 22-23-26-39-45 |
Components
| Standard component | α-Amylase, Heat-stable 10 mL |
| Amyloglucosidase 10 mL | |
| Celite 50 g | |
| Protease 500 mg |
